Complex DNA melting profiles of small PCR products revealed using SYBR Green I.

نویسندگان

  • Weijie Li
  • Biao Xi
  • Wancai Yang
  • Meredith Hawkins
  • Ulrich K Schubart
چکیده

1.Fire, A., S. Xu, M. K. Montgomery, S.A. Kostas, S.E. Driver, and C.C. Mello. 1998. Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans. Nature 391:806-811. 2.Sharp, P.A. 2001. RNA interference—2001. Genes Dev. 15:485-490. 3.Elbashir, S.M., W. Lendeckel, and T. Tuschl. 2001. RNA interference is mediated by 21and 22-nucleotide RNAs. Genes Dev. 15: 188-200. 4.Martens, H., J. Novotny, J. Oberstrass, T.L. Steck, P. Postlethwait, and W. Nellen. 2002. RNAi in Dictyostelium: the role of RNA-directed RNA polymerases and double-stranded RNase. Mol. Biol. Cell 13:445-453. 5.Malhotra, P., P.V. Dasaradhi, A. Kumar, A. Mohmmed, N. Agrawal, R.K. Bhatnagar, and V.S. Chauhan. 2002. Double-stranded RNA-mediated gene silencing of cysteine proteases (falcipain-1 and -2) of Plasmodium falciparum. Mol. Microbiol. 45:1245-1254. 6.Folk, P., F. Puta, L. Krpejsova, A. Blahuskova, A. Markos, M. Rabino, and R.P. Dottin. 1996. The homolog of chromatin binding protein Bx42 identified in Dictyostelium. Gene 181:229-231. 7.Skruzny, M., M. Ambrozkova, I. Fukova, K. Martinkova, A. Blahuskova, L. Hamplova, F. Puta, and P. Folk. 2001. Cyclophilins of a novel subfamily interact with SNW/SKIP coregulator in Dictyostelium discoideum and Schizosaccharomyces pombe. Biochim. Biophys. Acta 1521:146-151. 8.Morio, T., H. Urushihara, T. Saito, Y. Ugawa, H. Mizuno, M. Yoshida, R. Yoshino, B.N. Mitra, et al. 1998. The Dictyostelium developmental cDNA project: generation and analysis of expressed sequence tags from the first-finger stage of development. DNA Res. 5:335-340.

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عنوان ژورنال:
  • BioTechniques

دوره 35 4  شماره 

صفحات  -

تاریخ انتشار 2003